Student: Srushti Kolekar
Committee: Dr. Kuei-Chun Wang
Abstract:
To develop polymeric nanoparticles camouflaged in the leukocyte cell membrane for targeted delivery of DNA and/or small RNAs to sites of vascular injury and atherosclerotic lesions. Polymeric cores with DNA oligos as payload by a modified double emulsion method with poly D, L-lactic-co-glycolic acid were prepared and the plasma membrane of cultured Jurkat cells were collected. The polymeric cores were then be mixed with the isolated cell membrane and sonicated to create the leukocyte membrane-cloaked nanoparticles. Characterization of the physicochemical properties of the resulting nanoparticles with a dynamic light scattering (DLS) equipment and transmission electron microscopy was done. The loading efficiency of DNA oligos and the release profile were examined by a ssDNA assay kit with the Qubit fluorometer and confirmed by polymerase chain reaction. We expect that in conclusion the leukocyte membrane-coated nanoparticles will serve as biocompatible and efficient vehicles to deliver genes into target vascular cells.
Zoom Room: https://asu.zoom.us/j/8364458330
Presentation Time: 12:00-1:00 PM (Arizona Time)
